Water extracts of propolis collected from three geographic regions (Motobes, Kafr El-Sheikh and Desouk) in Kafr El-Sheikh Governorate, Egypt were prepared. The extracts were analyzed for the determination of total polyphenols which ranged from 5.70 to 8.79 g/100 g of the sample and from 22.80 to 34.30 g/100 g of the freeze-dried extract. the total flavonoidcontentrangedfrom3.05to4.85g/100gofthesample.Waterextractsofpropoliswereevaluatedfor antioxidant activities using the β- carotene bleaching and 1,1-diphenyl- 2- picrylhydrazyl (DPPH) free radical- scavenging assay systems. It was observed that all propolis had strong antioxidant activities due to their contents of total polyphenol and flavonoid.ThehighestactivitieswerefoundforsamplesfromDesoukfollowedbythesefromKafr El-Sheikh, then those from Motobes. Freeze-dried extracts of propolis can be used as natural antioxidants in sunfloweroilascomparedtoBHTandTBHQ.PropolisfromDesoukandKafrEl-Sheikhat200and300ppmweresimilar in reducing peroxide values and both of them at 300 ppm were better than BHT but lower than TBHQ, added at 200 ppm concentration, in reducing peroxides and hydroperoxides production in sunfloweroilat63°Cfor4days