In case there is no availability for photoelectric scanners for measuring the area spot of any lipid material on TLC plates, pixel area scanning technique with Total Lab software version 1.11 can replace the former. In order to test this argument, different lipid blends were interestrified with different lipases and the degree of synthesis was detected by determining the remaining triglycerides by TLC and pixel area scanning and Total Lab software version 1.11 as compared with column chromatography and gravimetry.