Background: Clinical supervision, a fundamental skill for head nurses, is based on developing head nurses' professional knowledge, skills, and professionalism, all of which are integral parts of professional identity. Aim: The present study aimed to examine the effect of a clinical supervision-enhancing strategy for head nurses on their professional identity. Material and methods: This study was a quasi-experimental research design (pre- and post-intervention) involving a convenience sample of 41 head nurses and 263 staff nurses working in Main Mansoura University Hospital. Data collection tools: The Self-administered Clinical Supervision Knowledge Questionnaire, Manchester Clinical Supervision Scale, and Professional Identity and Values Scale were used. Results: There was a statistically significant difference between head nurses' knowledge pre- and post-intervention. Also, there was a statically significant difference in head nurse clinical supervision effectiveness after the program implementation compared to before implementation. Conclusion: The program had a significant effect on all professional identity domains. The program had a large effect on total professional identity post-intervention and a medium effect during the follow-up phase. Recommendations: Educational programs can upgrade the level of professional performance and professional identity. Also, more researches are recommended in this area.