Background: Objective Structured Clinical Examination offers an attractive option for assessment of practitioner competency and evaluates in a comprehensive, consistent and structured manner, with close attention to the objectivity of the process and in more recent years have been increasingly utilized to assess nursing students. The aim of this study was to explore the undergraduate nursing students' perception and feedback about OSCE at the Maternity and Pediatric Nursing departments in the Faculty of Nursing, as an assessment tool for the clinical skills. Subject and Methods: Design: A descriptive exploratory study design was utilized. Setting: The study was conducted in the Faculty of Nursing, Port- Said University. Sample: A purposeful sample of 93 male and female nursing students on third year, who completed their maternity nursing as well as pediatric nursing OSCE examinations, was recruited for the study. Tool for data collection: A self-administered structured questionnaire was utilized to test the perception and feedback of the students. Results: The present study finding represents that the highest total mean score of OSCE was for evaluation subscale (25.6 ± 5.4), followed by a total mean score of quality of OSCE,(21.5 ±4.8) however, the lowest mean score (9.4± 2.3) was for the quality of student performance. Less than two third of the students (60.2% & 61.3%) agreed that OSCE covered a wide range of clinical skills, and the instructions of exam are clear and enough as information respectively. The OSCE provided opportunities to learn real life scenarios were seen by 61.3% of students. Conclusion and recommendations: Nursing students provided positive feedback about the OSCE and agreed that it was a realistic assessment for the practical courses. A broader research is necessary for assessment of factors affecting their perception regarding OSCE. Results will be applied to better understanding of this perception that can improve strategies for better OSCE.