Background: Probiotics are "live bacteria that, when provided in suitable amounts, impose a health benefit on the host," according to the World Health Organization. Objective: This study aims to assess the probiotic properties of Enterococcus bacteria and the safety and effectiveness of it as a probiotic agent.
Methods: Lactic acid bacteria, were isolated, using MRS media. Through biochemical tests, and PCR and sequencing methods the isolates appeared to be members of the Enterococcus species. So blood agar was used to grow them as a safety test. The presence of bacteriocin was then verified in the bacteria using a partial purification process. Its autoaggregation, hydrophobicity, biofilm formation, and other probiotic characteristics of the bacteria were investigated.
Result: When cultivated on blood agar media, the lactic acid bacteria were not hemolytic. The probiotics' characteristics included substantial capacity for auto aggregation, hydrophobicity, and biofilm formation, The results showed that the Etetrococcus employed in this study had bacteriocin, a pectocin variant effective against a variety of dangerous bacterial species.
Conclusion: Although it is not hemolytic when growing on blood agar medium, Enterococcus spp. isolated from conventional cheese and waterfowl intestines, in this study are unsatisfactory because of this safety trait. One of the properties of bacteria that makes them advantageous is that they produce bacteriocin and inhibit the growth of gram-negative bacteria.