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18049

Studying the Risks of Cotton Cooking Oil Fumes on the Lung and Liver of Rats and the Protective Role of (Vitamin E Alphatocopherol )

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Last updated: 03 Jan 2025

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Abstract

Fumes emitted from edible vegetable cooking oils during stir- and deep-frying are important contributors to indoor air pollution. Indoor air pollution may be increase the lung and liver cancer in Egypt in 2005. Therefore, the present study was undertaken to investigate the effects of Egyptian cotton cooking oil fumes on rats. The exposed rats for 30 and 60 days to cotton oil fumes showed a significant increase in the lung and liver malondialdehyde levels which accompanied with a significant decrease in glutathione content. Also, there was a significant decrease in lung and liver superoxide dismutase, catalase, glutathione s-transferase activities. As well as, there was a significant decrease in serum amino acids levels, lung and liver nucleic acids and total proteins. These changes were obviously after 60 days than that of 30 days of exposure. DNA change was clear in the lungs of rats after cotton oil fumes exposed as showed by the differential display technique, P53 primer which used to study the expression of the p35 gene as well as to confirm and amplify these changes after oil fumes exposure. Band with different molecular weights were observed after the exposure and in protected groups but not presented in the control. More characterization for the changes had been carried out in the animal on two levels, one the DNA using RAPD-PCR and the other on the protein level using SDS-PAGE techniques. Also, vitamin E ameliorates these abnormalities to extent limit in all cases in this work.

DOI

10.21608/ejhm.2005.18049

Keywords

cooking oil fumes, cotton oil, Polycyclic aromatic hydrocarbons (PAHs), superoxide dismutase (SOD), catalase (CAT), Glutathione S-transferase (GST), glutathione (GSH), lipid peroxidation (MDA), amino and nucleic acids, SDS- PAGE, RAPD-PCR, Differential Display, Vitamin E, rat, lung, Liver

Authors

First Name

Fahmy GAD GAD

Last Name

ELsaid

MiddleName

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Affiliation

Zoology Department, Faculty of Science, Mansoura University, Mansoura, Egypt.

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Volume

21

Article Issue

1

Related Issue

3769

Issue Date

2005-10-01

Receive Date

2018-10-31

Publish Date

2005-10-01

Page Start

53

Page End

65

Print ISSN

1687-2002

Online ISSN

2090-7125

Article File

EJHM_Volume 21_Issue 1_Pages 53-65.pdf

PDF . 443.9kB

Link

https://ejhm.journals.ekb.eg/article_18049.html

Detail API

https://ejhm.journals.ekb.eg/service?article_code=18049

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5

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Original Article

Type Code

606

Publication Type

Journal

Publication Title

The Egyptian Journal of Hospital Medicine

Publication Link

https://ejhm.journals.ekb.eg/

MainTitle

Studying the Risks of Cotton Cooking Oil Fumes on the Lung and Liver of Rats and the Protective Role of (Vitamin E Alphatocopherol )

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Article

Created At

22 Jan 2023