Fluorescence spectroscopy is a technique used to determine fluorescence spectrum that emits by fluorescent compounds. Milk had more than one fluorescent compounds such as tryptophan, vitamin A and etc. Quick identification of milk types is needed in dairy plants for milk quality control and safety. The ability of two different fluorescence spectroscopy techniques (front face fluorescence spectroscopy and synchronous scanning fluorescence spectroscopy) was determined to discriminate 40 milk samples according to milk species (10 samples for each of buffalo's, cow's, goat's and sheep's milk). The statistical methods, principal component analysis PCA and factorial discriminant analysis FDA, were used for more illustrating the obtained results. FDA was applied separately on the first five principal components obtained from PCA which performed on the two different fluorescence techniques. Results obtained from FDA showed that 100% of correct classification was obtained for data sets from the two different fluorescence techniques. The obtained results confirmed that the front face fluorescence spectroscopy and synchronous scanning fluorescence spectroscopy were capable of differentiate milk species.