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157909

Prevalence of enterotoxigenic Staphylococcus aureus with characterization of enterotoxins for subsequent detection

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Last updated: 03 Jan 2025

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Abstract

In this work 68 S. aureus isolated frommastitic cows (n=48), camel milk (n=5) and human samples (urine n = 4, abscesses aspiration n = 3, sputum n = 2, nasal swabs n = 2, vaginal swabs n = 1 and wound swabs n = 3) were investigated using phenotypic and molecular identification to detect the occurrence of enterotoxins. Eighteen out of 48 S. aureus isolated from mastitic cows and 5 out of 15 S. aureus isolated from human samples were enterotoxin producers. All toxigenic S. aureus isolated from clinical mastitic cows (13) were toxin type D producer while seb and sed were detected from subclinical mastitic cows. Among human isolates, 2 isolates produced mixed toxins A and E, 2 isolates produced mixed toxins C and D and one isolate produced type C toxin. No toxin was detected from camel isolates. All toxigenic S. aureus isolated from cows (13) were toxin type D producer. Among human isolates 2 isolates produced mixed toxins A and E, 2 isolates produced mixed toxins C & D and one isolate produced type C toxin.  Five S. aureuscrude enterotoxin type D were investigated by protein SDS-PAGE. It was clear that all isolates had from 9 to 7 bands.  All the isolates had a band at 148-159 kDa, 35-42 kDa, 28-30 kDa, 23-26 kDa, 20-22 kDa and 18-19 kDa. 4 isolates had a band at 48-55 kDa, 3 isolates had a band at 39-42 kDa, 3 isolates had a band at 31-34 kDa and one isolate had a band at 17 kDa.

DOI

10.21608/vmjg.2020.157909

Keywords

enterotoxin, Multiplex PCR, Mastitic milk, S. aureus, SDS

Authors

First Name

Fatma Elzahraa

Last Name

Mohamed

MiddleName

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Affiliation

Department of Microbiology, Faculty of Veterinary Medicine, Cairo University, Giza.

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Orcid

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First Name

Omnia

Last Name

H

MiddleName

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Affiliation

Animal Health Research institute, Giza, Egypt.

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City

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Orcid

-

First Name

Heba

Last Name

Deif

MiddleName

-

Affiliation

Department of Microbiology, Faculty of Veterinary Medicine, Cairo University, Giza.

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City

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Orcid

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First Name

Eman

Last Name

Ragab

MiddleName

-

Affiliation

Department of Microbiology, Faculty of Veterinary Medicine, Cairo University, Giza.

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City

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Orcid

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First Name

Jakeen

Last Name

Eljakee

MiddleName

-

Affiliation

Department of Microbiology, Faculty of Veterinary Medicine, Cairo University, Giza.

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Volume

66

Article Issue

1

Related Issue

23198

Issue Date

2020-12-01

Receive Date

2020-04-15

Publish Date

2020-12-01

Page Start

98

Page End

114

Print ISSN

1110-1423

Online ISSN

2537-1045

Link

https://vmjg.journals.ekb.eg/article_157909.html

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https://vmjg.journals.ekb.eg/service?article_code=157909

Order

9

Type

Original Article

Type Code

544

Publication Type

Journal

Publication Title

Veterinary Medical Journal (Giza)

Publication Link

https://vmjg.journals.ekb.eg/

MainTitle

Prevalence of enterotoxigenic Staphylococcus aureus with characterization of enterotoxins for subsequent detection

Details

Type

Article

Created At

22 Jan 2023