Plants belonging to the Genus Passiflora have been commonly used in traditional medicine for a variety of health conditions. The major bioactive components present in these plants are identified as flavonoids, alkaloids, glycosides and phenolic compounds. Six species belonging to the Genus Passiflora which have been well documented are P. alata, P. caerulea, P. edulis, P. foetida, P. incarnata, P. ligularis. Also, the subspecies P. edulis Sims f. Flavicarpa has been well documented for its phytochemical composition and human healthcare applications. There are well documented evidence-based scientific studies which are continuing to be reported on the medicinal value of these plants. These studies include both in vitro and in vivo studies using cell lines and animal models, respectively. The studies highlight the importance of these plant species for their therapeutic values in gastrointestinal conditions, neurological complications, cardiovascular conditions, inflammation and as anti-anxiolytic agents. We present in this review a systematic account on the phyto-constituents present in various plant parts of the six different species of this Genus along with the in vitro and in vivo experiments that highlighted their pharmacological importance.