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188692

Next Day Detection of Salmonella spp. In Naturally Contaminated Meat Products Using Molecular Approach

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Last updated: 03 Jan 2025

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Abstract

Salmonella species are a well known food borne pathogen in developed and developing countries with a wide variety of illnesses conditions. The aim of this study was to compare a traditional isolation and identification method for Salmonella spp from meat products with a newer DNA/rRNA gene hybridization method [LUMIprobe 24 Salmonella] under the routine food laboratory conditions. A total of 133 samples [30 pork sausage, 40 chicken meat coated with oil and spices, 40 kebab and 23 beef burger] were collected from February to May 2004, samples were processed according to the guideline of the British Standard Cultural Method; BS EN 12824:1998, as well as, the DNA/rRNA method as guided by the manufacture. Results showed that the two methods gave equivalent sensitivity and selectivity for Salmonella spp detection. Significant time saving was achieved using the LUMIprobe 24 test allowing earlier release of results on the next day and the new molecular method experienced a reasonable level of flexibility and labor saving.

DOI

10.21608/jhiph.2004.188692

Keywords

Salmonella spp, Naturally Contaminated Meat Products, molecular approach

Authors

First Name

Ihab

Last Name

Habib

MiddleName

E.

Affiliation

Food Hygiene and Control Division, The High Institute of Public Health, University of Alexandria, Egypt

Email

habib@murdoch.edu.au

City

-

Orcid

-

Volume

34

Article Issue

2

Related Issue

26024

Issue Date

2004-04-01

Receive Date

2021-08-10

Publish Date

2004-04-01

Page Start

279

Page End

288

Print ISSN

2357-0601

Online ISSN

2357-061X

Link

https://jhiphalexu.journals.ekb.eg/article_188692.html

Detail API

https://jhiphalexu.journals.ekb.eg/service?article_code=188692

Order

2

Type

Original Article

Type Code

511

Publication Type

Journal

Publication Title

Journal of High Institute of Public Health

Publication Link

https://jhiphalexu.journals.ekb.eg/

MainTitle

Next Day Detection of Salmonella spp. In Naturally Contaminated Meat Products Using Molecular Approach

Details

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Article

Created At

22 Jan 2023