A total of 125 random samples of raw milk and soft cheese (25 each) of cow's milk, sheep's milk, goat's milk, kareish cheese and Domiati cheese samples were collected from different markets and shops in Alexandria city, Egypt and examined for the presence of Staphylococcus aureus and Streptococcus agalactiae as food poisoning and mastitis causing organisms. The incidence of Staphylococcus aureus in examined samples were 28, 36, 40, 20 and 16% in the examined cow's milk, sheep's milk, goat's milk, kareish cheese and Domiati cheese, respectively.  Streptococcus agalactiae was detected in 16, 20, 24, 12 and 4% of the examined samples, respectively. Streptococcus agalactiae was identified using primers V1 and V2, specific to rRNA as an early diagnosis of subclinical mastitis using, PCR technique. The sanitary and public health importance of these organisms as well as control measures to improve the quality of dairy products and to safeguard the consumers from infection were discussed.