Humans are exposed to hepatocarcinogenic aflatoxins (Afs) through ingestion of contaminated foods as a result of poor storageful of susceptible grains or eating foods contaminated with aflatoxins animals and vegetables. This work aimed at evaluating the effect level of total (Afs) on human health. Study samples ninety persons divided into three groups (each /30 human). Group (1) no suffering liver disease as normal or negative control. Group (2) patients input (El Raghi hospital in Assuit) are suffering of liver inflammatory disease (HI). Group (3) patients input/or output (El Raghi hospital in Assuit) are suffering of hepatocellular carcinoma (HCC). Random samples were obtained from some foods (chicken egg; chicken, duck and rabbit liver) purchased from local market in Assuit. Total amount of aflatoxins was determined by using high-performance liquid chromatography (HPLC) instrumental. This study used 24 h recall and food frequency questioners for these groups to determination the type and amount of food which were intake. In serum humans measured total aflatoxins by used the enzyme-linked immunosorbent assay (ELISA) kits and ELISA technique. In addition oxidation tests are carried out for the studied individuals by using colorimetric kites to determine liver function (Alanine transaminase (ALT), Aspartate transaminase (AST), Total protein (TP),albumin (Alb), Bilirubin (Total, Direct), alkaline phosphatase (ALP), Glutathione peroxidase (GPX) and Malondialdehyde (MDA) by used spectroscopy instrument. Results indicated that were significant increase in liver function and biomarker antioxidants (GPX, MDA). Study concluded that these alterations in liver functions could be related to the development of liver damage in response to significant dose of Aflatoxins and this work aimed at evaluating the effect level of total (Afs) on human health.