Purpose: This study aimed to compare the antibacterial effect and microleakage of two types of fissure sealants; Beautisealant and Embrace wet Bond. Materials and Methods: for evaluation of the antibacterial effect, 30 saliva samples were obtained and were sent to microbiology laboratory where streptococcus mutans were isolated and the antibacterial effect of both materials was detected by the agar diffusion method. For microleakage test 30 extracted sound permanent molars were obtained. Two groups were formed (Group A &Group B) 15 teeth each. Teeth in group A were sealed with Embrace wet bond while Beautisealant was used to seal teeth in group B. Dye penetration was performed then specimens were put for 24 hours in 2% buffered methylene blue dye. Resin blocks were prepared then longitudinally sectioned buccolingually, The specimens were assessed for dye penetration with digital microscope. Results: For antibacterial effect it was found that Embrace wet Bond recorded higher bacterial inhibition zone than Beautisealant. Mann-Whitney-test (P=<0.0001< 0.05) was used to determine the difference between both groups and showed statistical significance. For the microleakage test it was found that Beautisealant recorded higher value of leakage than Embrace wet Bond. The difference between both groups showed statistical non-significance as determined by Mann-Whitney-test (P=0.4> 0.05).Conclusion: Embrace wet bond pit and fissure sealant could be better option than Beautisealant regarding the antibacterial effect however no difference was noticed between them regarding the microleakage.