This study was designed to investigate the effects of consumption acidic and alkaline food to determine offspring gender. In total, 20 female albino rats and 5 males were chosen and randomly divided into 5 groups all groups of rats were fed on the experimental diet. After 20 days of special diet, the female rats were mated with males and were separated after pregnancy. The newborn rats' sexes were determined after delivery. In potatoes group (58%) males and (42%) female rats were born (sex ratio = 1.38) while these rates were (61%) male and (39%) female (sex ratio = 1.56) in potatoes plus K2HPO4 group , in milk group (57%)male and (43%) females (Sex ratio = 1.33), in milk plus CaCO3 (46%)male and (54%) female (Sex ratio = .85) while in control group (47%) male and (53%) female rats were born (sex ratio = .89). (ALT), and (AST) were determined, Serum creatinine, serum uric acid and serum bilirubin measured. Blood analysis included WBC count, and platelet count (PLC). The present results observed that acidic and alkaline food in the diet of rats can have significant effect on the sex ratio of delivered rats' offspring.