This study was carried out onOriganum vulgare L. plant during the twosuccessive seasons of 2013 and 2014, at the Experimental Station ofMedicinal and Aromatic Plants of Sekem Company, Belbis desert- ELSharkiya, Egypt. The aim of this research was to study the effect of thecombination between compost, biofertilizer and algae extract on the growth, productivity and essential oil production ofOriganum vulgare L. plants. Theorganic fertilization of Sekem compost was added at rates of 5, 10 and15m3/fed. A mixture of equal percentages of five strains of bacteria namely, Azotobacter chroococcum, Azospirillum lipoferum (as nitrogen fixing bacteria),Bacillus polymixa , Bacillus megatherium and Pseudomonas fluorescence (asphosphate solubilizing bacteria) was used as a source of biofertlizer. Thebiofertilizer a mixture at concentration of 1x108 ml was obtained fromSekem Co, Egypt. Algae extract was obtained from Algae Production Unit atthe National Research Centre. It was an extract of the algae  Spirulinaplatensis. The results showed that, the highest significant increment forgrowth and yield characters was resulted from the treatment of algae extract +biofertilizer + 15m3 compost this combination treatment resulted in the highestmean value of oil yield (ml/ plant). As general the main chemical constituentsof oregano essential oil were carvacrol, p-cymene, ç-terpinene,α-thujene, α-myrcene,α-terpinene, o-cymene, carvacrol methyl ether andcaryophyllene.