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Phenolic Compounds and Hepatoprotective Activity of Centaurea aegyptiaca L. on Carbon Tetrachloride-induced Hepatotoxicity in Rats

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Last updated: 22 Jan 2023

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Abstract

Objectives: This study aimed to isolate phenolic compounds from the 70% aqueous methanol extract of the aerial parts (leaves, flowers and stems) of Centaurea aegyptiaca , evaluate in-vivo hepatoprotective activity and determine total phenolic and total flavonoid contents. Methods: 70% aqueous methanol extract of the aerial parts subjected to different chromatographic separation techniques. The identities of the isolated compounds were established on the basis of their spectral data and comparing with previously reported data. The crude extract was evaluated for in-vivo hepatoprotective activity on CCl4-induced hepatotoxicity in rats. Total phenolic and total flavonoid contents of the aqueous methanol extract were determined using Folin-Ciocalteu and aluminum chloride colorimetric methods, respectively. Results: The aqueous methanol extract of the aerial parts of Centaurea aegyptiacaafforded seven compounds. A phenolic acid ester; protochatechuic acid methyl ester (1) along with six known flavonoids; apigenin-6-C-β-D-glucopyranoside (isovitexin) (2), apigenin-8-C-β-D-glucopyranoside (vitexin) (3), quercetin-3-O-b-D-glucopyranoside (isoquercetin) (4), apigenin (5), 3-O-methylquercetin (6) and quercetin (7). Administration of the extract at doses of 100 and 200 mg/kg b.wt showed a hepatoprotective activity similar to that of the standard drug; silymarin at a dose of 25 mg/kg b.wt. Comparative histopathological study of liver exhibited moderate changes in liver histoarchitecture when compared to the CCl4 group. The methanolic extract showed high concentration of phenolic and flavonoid contents. Conclusion: The aqueous methanol extract of the aerial parts of C. aegyptiaca afforded seven phenolic compounds for the first time from this species, with promising in-vivo hepatoprotective activity.

DOI

10.21608/aprh.2018.5890

Keywords

Asteraceae, Centaurea aegyptiaca , Flavonoids, Hepatoprotective

Authors

First Name

Wessam

Last Name

Senosy

MiddleName

-

Affiliation

Pharmacognosy Department, Faculty of Pharmacy, MUST University, 6th October City, Giza, Egypt

Email

wessamfirst@gmail.com

City

-

Orcid

-

First Name

Amel

Last Name

Kamal

MiddleName

-

Affiliation

Pharmacognosy Department, Faculty of Pharmacy, Helwan University, Helwan City, Cairo, Egypt

Email

kh.omran@yahoo.com

City

-

Orcid

0000-0002-9803-7404

First Name

Sayed

Last Name

El-Toumy

MiddleName

-

Affiliation

Chemistry of Tannins Department, National Research Centre, 12622 Dokki, Cairo, Egypt

Email

-

City

-

Orcid

-

First Name

El.Husseiny

Last Name

El.Gendy

MiddleName

-

Affiliation

Pharmacognosy Department, Faculty of Pharmacy, MUST University, 6th October City, Giza, Egypt

Email

-

City

-

Orcid

-

Volume

2

Article Issue

2

Related Issue

1044

Issue Date

2018-04-01

Receive Date

2017-12-21

Publish Date

2018-04-01

Page Start

123

Page End

132

Print ISSN

2357-0547

Online ISSN

2357-0539

Article File

APRH_Volume 2_Issue 2_Pages 123-132.pdf

PDF . 1.1MB

Link

https://aprh.journals.ekb.eg/article_5890.html

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https://aprh.journals.ekb.eg/service?article_code=5890

Order

6

Type

Research Article

Type Code

318

Publication Type

Journal

Publication Title

Journal of Advanced Pharmacy Research

Publication Link

https://aprh.journals.ekb.eg/

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Article

Created At

22 Jan 2023