Biscuits are favored products for people of different ages world-wide due to its high-energy foods, long shelf-life, and easy absorption. This study aimed to evaluate the fortified biscuit with iron nanoparticles (Fe2O3-NPs) as novel approach to overcome iron deficiency anemia (IDA) disease. In preliminary experiments, we utilized 4 groups of albino rats as follows; negative group (G1; control), positive group (G2), treated anemic group (G3), and untreated anemic group (G4). The SEM, XRD, histopathological, and hematology tests were performed. The results revealed that the Fe2O3-NPs was at 49.5±5 nm, homogenous, and purity. Fortified biscuits with Fe2O3-NPs (10 ppm Fe2O3-NPs per 20g biscuit) increased the iron level (289±1.0 mg/dL), red blood counts (9.1±0.2 1012/L), and hemoglobin (18±0.25 g/dL) in the G3 compared to other groups. Histological examination showed that no significant effects on the spleen, kidney, and liver. Results demonstrated that the fortified biscuit with Fe2O3-NPs (10 ppm) at 49.5±5 nm is more effective in IDA treatment.