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228804

The Impact of Different Conditions on Protease Hydrolysis for Gliadin Degradation

Article

Last updated: 01 Jan 2025

OverviewSimilar Items

Subjects

-

Tags

Analytical chemistry

Abstract

α-gliadin has been proven to be the cause of gliadin's toxicity for celiac patients because it is highly immunogenic. Therefore, the effect of Aspergillus niger protease on gliadin degradation under different conditions (concentration, pH, incubation time, and temperature) was carried out. A sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) test revealed the ability of fungal protease to degrade gliadin. According to the results, the optimal conditions to completely degrade α-, β-, γ-, and ω-gliadin were 0.3 g fungal enzyme/100 g wheat flour under pH 3 for 16 hours at 55 °C.

DOI

10.21608/ejchem.2022.118163.5324

Keywords

Wheat gluten, Gliadin degradation, Aspergillus niger, Protease, SDS-PAGE

Authors

First Name

Sayed

Last Name

Mostafa

MiddleName

-

Affiliation

Food Technology Dep, Food industries and Nutrition Institute, National Research Centre.

Email

abnody.sayed@gmail.com

City

Giza

Orcid

0000-0002-3770-8024

First Name

‫الأبنودى‬‎

Last Name

Faheid

MiddleName

-

Affiliation

Department of food science, National Research Center, Giza, Egypt

Email

s_faheid@yahoo.com

City

Giza

Orcid

0000-0002-1692-4174

First Name

Ibrahim

Last Name

Rizk

MiddleName

-

Affiliation

Food Science Department, Faculty of Agriculture, Ain Shams University

Email

ibrahimrizk@gmail.com

City

-

Orcid

-

First Name

‫الأبنودى‬‎

Last Name

Ragab

MiddleName

H

Affiliation

Food Technology Department, National Research Center, Giza, Egypt.

Email

gragab@yahoo.com

City

Giza

Orcid

0000-0002-7332-6285

First Name

Yasser

Last Name

Kishk

MiddleName

-

Affiliation

Food Science Department, Faculty of Agriculture, Ain Shams University

Email

yasserkishk@gmail.com

City

-

Orcid

-

Volume

65

Article Issue

13

Related Issue

37459

Issue Date

2022-12-01

Receive Date

2022-01-24

Publish Date

2022-12-01

Print ISSN

0449-2285

Online ISSN

2357-0245

Link

https://ejchem.journals.ekb.eg/article_228804.html

Detail API

https://ejchem.journals.ekb.eg/service?article_code=228804

Order

228,804

Type

Original Article

Type Code

297

Publication Type

Journal

Publication Title

Egyptian Journal of Chemistry

Publication Link

https://ejchem.journals.ekb.eg/

MainTitle

The Impact of Different Conditions on Protease Hydrolysis for Gliadin Degradation

Details

Type

Article

Created At

22 Jan 2023