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The evaluation of antioxidant and anti-inflammatory activities of Parmotrema hypotropa lichen extract

Article

Last updated: 01 Jan 2025

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Subjects

-

Tags

Biochemistry

Abstract

Lichens are used since antiquity for multiple therapeutic purposes, treating infections, fever, convulsions, and wounds. This study was designed to evaluate the antioxidant and anti-inflammatory effects of the lichen Parmotrema hypotropa (NYL.) Hale. The total phenolics and flavonoids content were determined in ethanol extract; the antioxidant activity was assessed by different chemical assays DPPH (1,1-diphenyl-2-picrylhydrazyl), ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)), β-carotene-linoleic acid, reducing power, and Cupric reducing antioxidant capacity (CUPRAC). The lichen extract was further tested in vitro, for its anti-inflammatory effect through the bovine serum albumin denaturation assay; also the inflammatory response was investigated on the LPS-stimulated human keratinocyte cell line (HaCaT) by measuring intercellular ROS (Reactive Oxygen Species) and TNF-α (Tumor necrosis factor-alpha) production; the amount of usnic acid in the extract was determined by High-Performance Liquid Chromatography (HPLC). The results showed that the tested extract contained high levels of total phenolics than flavonoids, and had an antioxidant effect; it prevented the protein denaturation in a dose-dependent manner, also reduced the intracellular ROS and TNF-α production by HaCaT cells. Furthermore, it did not affect the viability of HaCaT cells; the extract contained small amounts of usnic acid. Based on the different findings, P.hypotropa can constitute a potential source of bioactive compounds with antioxidant and anti-inflammatory effects.

DOI

10.21608/ejchem.2022.109501.5016

Keywords

Lichen, Parmotrema hypotropa, Antioxidant activity, anti-inflammatory effect, HaCaT cells, usnic acid

Authors

First Name

Maya Abir

Last Name

TARTOUGA

MiddleName

-

Affiliation

Department of Animal Biology, Faculty of Natural and Life Sciences, University Frères Mentouri Constantine 1, route Ain el bey 25000, Constantine Algeria.

Email

tartouga.abirmaya@umc.edu.dz

City

Constantine

Orcid

0000-0001-9692-5339

First Name

Ibtissem

Last Name

ELOUAR

MiddleName

-

Affiliation

Department of Animal Biology, Faculty of Natural and Life Sciences, University Frères Mentouri Constantine 1, route Ain el bey 25000, Constantine Algeria.

Email

louar.ibtissem@umc.edu.dz

City

-

Orcid

-

First Name

Ibtissem

Last Name

ZEGHINA

MiddleName

-

Affiliation

Department of Animal Biology, Faculty of Natural and Life Sciences, University Frères Mentouri Constantine 1, route Ain el bey 25000, Constantine Algeria.

Email

zeghina.ibtissem@umc.edu.dz

City

-

Orcid

-

First Name

Mohamed Badreddine

Last Name

MOKHTARI

MiddleName

-

Affiliation

Department of Animal Biology, Faculty of Natural and Life Sciences, University Frères Mentouri Constantine 1, route Ain el bey 25000, Constantine Algeria.

Email

mohamedbadreddine.mokhtari@umc.edu.dz

City

-

Orcid

-

First Name

Lynda

Last Name

GALI

MiddleName

-

Affiliation

National Center in Biotechnological Research (CRBT), 25 000 Constantine, Algeria.

Email

gali.lynda2@gmail.com

City

-

Orcid

-

First Name

Shara Francesca

Last Name

RAPA

MiddleName

-

Affiliation

Department of Pharmacy, School of Pharmacy, University of Salerno, Via Giovanni Paolo II 132, I-84084 Fisciano, Italy.

Email

s.rapa@studenti.unisa.it

City

-

Orcid

-

First Name

Nunziatina

Last Name

DE TOMMASI

MiddleName

-

Affiliation

Department of Pharmacy, School of Pharmacy, University of Salerno, Via Giovanni Paolo II 132, I-84084 Fisciano, Italy.

Email

detommasi@unisa.it

City

-

Orcid

-

First Name

Stefania

Last Name

MARZOCCO

MiddleName

-

Affiliation

Department of Pharmacy, School of Pharmacy, University of Salerno, Via Giovanni Paolo II 132, I-84084 Fisciano, Italy.

Email

smarzocco@unisa.it

City

-

Orcid

-

First Name

Chawki

Last Name

BENSOUICI

MiddleName

-

Affiliation

National Center in Biotechnological Research (CRBT), 25 000 Constantine, Algeria.

Email

chawkiislam@yahoo.fr

City

-

Orcid

-

Volume

65

Article Issue

9

Related Issue

31876

Issue Date

2022-09-01

Receive Date

2021-12-08

Publish Date

2022-09-01

Page Start

109

Page End

118

Print ISSN

0449-2285

Online ISSN

2357-0245

Article File

EJCHEM_Volume 65_Issue 9_Pages 109-118.pdf

PDF . 395.4kB

Link

https://ejchem.journals.ekb.eg/article_223227.html

Detail API

https://ejchem.journals.ekb.eg/service?article_code=223227

Order

11

Type

Original Article

Type Code

297

Publication Type

Journal

Publication Title

Egyptian Journal of Chemistry

Publication Link

https://ejchem.journals.ekb.eg/

MainTitle

The evaluation of antioxidant and anti-inflammatory activities of Parmotrema hypotropa lichen extract

Details

Type

Article

Created At

22 Jan 2023