Hyperlipidemia is a gear in a big cascade leading to fatal disease ending in death especially among elderly patients. The reduction in serum cholesterol may alter or save the human life away from cardiovascular risks. Due to what called “drug side effects" and the failure of modern medicine to control this issue. So, the use of natural products in remedy becomes the famous topic Raphanus sativus L. (Cruciferaceae), commonly known as radish. Radish, Raphanus sativus L., is an annual vegetable belonging to the family Cruciferae and is a traditionally important vegetable in many countries All plant parts are edible and it has been used in folk medicine. The present study was designed to evaluate phytochemical analysis and hypolipidemic activity of radish seed oil (RSO) in a hyperlipidemic rat model induced by a high-fat diet (HFD). GC/MS of the Radish oil seed fatty acid methyl esters revealed that the major fatty acids were unsaturated fatty acids, and saturated fatty acids (79%, 21 %) of total fatty acids respectively. Twenty-four rats were divided into three groups, group I as control, group II fed with high-fat diet (HFD), and group III fed with HFD and administered RSO orally (70 μl/Kg) for ten weeks. Results showed that Raphanus sativus seed oil significantly inhibits the body weight gain of rats induced by HFD, significantly reducing concentration levels of plasma cholesterol, triglycerides, and LDL- cholesterol in hyperlipidemic rats. Conclusion Raphanus sativus seed oil has good hypolipidemic effects on hyperlipidemic rats thus suggesting its beneficial effect in the treatment of cardiovascular diseases associated with hyperlipidemia.