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198014

Statistical, Optimization, And Thermodynamic Studies On The Production Of Alkaline Protease Using New Local Isolate Of Bacillus Sp

Article

Last updated: 22 Jan 2023

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Subjects

-

Tags

Organic chemistry

Abstract

Abstract
Investigation of alkaline protease production under different fermentation conditions using one factor at a time involving temperature, pH, incubation time as well as different carbon and organic nitrogen sources .The results showed that the maximum activity (470U/ml) was obtained at peptone 7.5 (g/L), glucose 5 (g/L), pH 10, 24h incubation time and 35oC. Further more, Two sequential statistical designs were used to optimize these physiochemical parameters (pH, temperature, incubation time, glucose, peptone, and MgSO4.7H2O, K2HPO4) for enzyme production. Firstly, the Plackett Burman factorial design was applied followed by Box-Behnken experimental design. The results showed that the best protease production activity (580 U/ml) was obtained at peptone 10 (g/L), glucose 12.5 (g/L), pH 11, 24h incubation time and 35oC. The level of significance was studied using the three significant variables on the enzyme production (pH, glucose and peptone conc.) and the results indicated the integration of these parameters on protease production. The thermodynamic parameters of crude enzyme were also investigated and they proved the superior thermal stability of the B. amyloliquefaciens protease .

DOI

10.21608/ejchem.2021.94682.4454

Keywords

Alkaline protease, Bacillus, Statistical design, Thermodynamics

Authors

First Name

Olfat E.

Last Name

Amin

MiddleName

-

Affiliation

Chemistry of Natural and Microbial Products Department, Pharmaceutical and Drug Research Division , National Research Centre, El-Behooth St., Dokki, Giza, Egypt.

Email

-

City

-

Orcid

-

First Name

Ahmed M.

Last Name

Aboul-Enein

MiddleName

-

Affiliation

Biochemistry Department, Faculty of Agriculture, Cairo University, Egypt.

Email

abuelenien@yahoo.com

City

cairo

Orcid

-

First Name

Ibrahim S.

Last Name

Abd-Elsalam

MiddleName

-

Affiliation

Chemistry of Natural and Microbial Products Department, Pharmaceutical and Drug Research Division , National Research Centre, El-Behooth St., Dokki, Giza, Egypt.

Email

abdelsalam66@hotmail.com

City

Cairo

Orcid

-

First Name

Marwa I.

Last Name

Wahba

MiddleName

-

Affiliation

Chemistry of Natural and Microbial Products Department, Pharmaceutical and Drug Research Division , National Research Centre, El-Behooth St., Dokki, Giza, Egypt., Centre of Scientific Excellence-Group of Advanced Materials and Nanotechnology, National Research Centre, El-Behooth St., Dokki, Giza, Egypt

Email

dmarwawahba@yahoo.com

City

cairo

Orcid

-

First Name

Heba A.

Last Name

El-Refai

MiddleName

-

Affiliation

Chemistry of Natural and Microbial Products Department, Pharmaceutical and Drug Research Division , National Research Centre, El-Behooth St., Dokki, Giza, Egypt.

Email

dr.heba_ar@yahoo.com

City

cairo

Orcid

-

Volume

65

Article Issue

4

Related Issue

29683

Issue Date

2022-04-01

Receive Date

2021-09-07

Publish Date

2022-04-01

Page Start

301

Page End

313

Print ISSN

0449-2285

Online ISSN

2357-0245

Article File

EJCHEM_Volume 65_Issue 4_Pages 301-313.pdf

PDF . 530.9kB

Link

https://ejchem.journals.ekb.eg/article_198014.html

Detail API

https://ejchem.journals.ekb.eg/service?article_code=198014

Order

29

Type

Original Article

Type Code

297

Publication Type

Journal

Publication Title

Egyptian Journal of Chemistry

Publication Link

https://ejchem.journals.ekb.eg/

MainTitle

-

Details

Type

Article

Created At

22 Jan 2023