Plant parasitic nematodes: Meloidogyne incognita, Pratylenchus penetrans, and Tylenchulus semipenetrans are the most genera which cause economic damage in the agricultural production. Nanotechnology is a promising alternative method in nematode control, especially the green synthesis methods. Present study deals with the effects of Ag engineered nanoparticles (AgENPs) synthesized by using plant extract to evaluate juvenile mortality, egg hatching, morphological change, and DNA damage. Five concentrations of AgENPs were prepared from each AgENPs and were added to300 individuals of nematode separately up to five days. Data approved that, Meloidogyne incognita was more affected than Pratylenchus penetrans and Tylenchulus semipenetrans in all the treatments. Curcuma comosa (N1) was the most effective preparation against nematode larvae, followed by Chitosan (N3), Crotalaria juncea (N4), and Cycas circinalis (N2). These effects were increased proportionally with the nano concentration and the exposure time. While N3 recorded the highest reduction in egg hatchability. Some visual changes were observed on the treated larvae and eggs. Likewise, the most DNA damage for nematode larvae was detected with N1,followed by N4, N3, and N2. As a conclusion, AgENPs may provide a better alternative to chemically synthesized against deferent plant parasitic nematode.