Pesticide residue has become as one of the major food adulterants as a result , rapid, simple, spectrophotometric method for pesticide residue measurement (bifenthrin, glyphosate) in milk sample based on UV-Vis spectrophotometer and Artificial neural network had been developed .The developed system detecting the milk contaminants like pesticide residue and classified it based on absorption spectrum. The hybrid system consists of UV-Vis spectrophotometer and neural package. The detection and classification processes depend on the readout of the UV-Vis spectroscopy to achieve the detection and recognition of all the samples by using Levenberg-Marquardt back propagation (LM-BP) neural network algorithm. The reported classification rate accuracy reached above 99.98%.