This work proved antioxidant and antimicrobial effects and chemical content of Cichorium intybus L. Methanol extract of C. intybus L. aerial parts was tested for antimicrobial activity and for antioxidant effect. The results showed that the extract has a significant antimicrobial activity, it inhibited the growth of the Gram-negative bacteria and Fungi (has registered against various). The extract has a good antioxidant activity reported to Vitamin C (IC50=61.57±0.75 µg/mL; Vitamin C=5.78±0.08 µg/mL). Phytochemical evaluation has shown that the plant has flavonoids, triterpenes and others, and further phytochemical proved the identification of β-sitosterol, apigenin, kaempferol, apigenin 3-O-α-rhamnoside, apigenin 3-O-β-glucoside, and kaempferol-3-O-β-glucoside. In conclusion, C. intybus can be a new promising antioxidant and antimicrobial agents.