The inorganic nano-sized silicon dioxide modify polyester fabrics using IR dyeing machine was constructed. Chemical activation method with alkali hydrolysis of the fabric was done to facilitate the binding affinity of SiO2 NPs via carboxylic and hydroxyl groups created on the fabric surface. The morphological structure of the treated fabrics was investigated by field-emission scanning electron microscopy (FE-SEM). Dispersive X-ray spectroscopy (EDX) of the untreated and treated polyester fabrics was measured. Fourier transformed infrared spectroscopy (FTIR) of the untreated and treated fabrics was elucidated. TEM of the used silicon dioxide nano particles was monitored. Thermogravimetric analysis (TGA) and UV protection and of the treated fabrics were studied. Antimicrobial activity of finished polyester fabrics containing SiO2 NPs was tested against Gram-positive (Staphylococcus aureus), Gram-negative (Escherichia coli), and pathogenic fungi (Candida albicans and Aspergillus flavus). The results indicate that the nano-silica has an excellent effect on antimicrobial activity and good protection of both UVA and UVB even after ten washing cycles, indicating the excellent laundering durability. The performance of treated fibers to be used as medical textile was determined by radar chart area.