The present work describes for the first time the fabrication of a novel disposable screen printed sensor for the potentiometric determination of trospium hydrochloride (TRO). The cited sensors were based on alpha-cyclodextrin as molecular recognition element and multi walled carbon nanotubes (MWCNTs) as transducer. Comprehensive and deep investigations were done on the sensing membrane components including the nature of sensing material, cavity size, additives, and plasticizers in addition to nanomaterial. The fabricated sensors were highly sensitive, selective and accurate towards TRO with Nernstian slope 60.8± 0.5mV decade-1in the concentration range from 10-6 to 10-2 molL-1. This due to the incorporation of MWCNTs to the electrode matrix which accelerates response time and improves potential reading stability. The fabricated sensors were used for the potentiometric determination of TRO under batch and flow injection technique with agreeable recoveries comparedwith the standard methods. Moreover, the high selectivity of the sensors towards TRO in presence of its degradation products suggests application of this method as stability indicating technique for TRO quality control.