This article was concentrated on the preparation of antimicrobial and protective cellulosic fabrics based polysaccharides material by natural macromolecule and carbohydrate biopolymer such as chitosan. This study also addressed the application of nanotechnology in achieving this purpose using silver nanoparticles (AgNPs). Chitosan and AgNPs were also used in the treatment bath to improve some functional properties of these fabrics. Some investigations were performed for the treated fabrics such as ultra violet protection factor (UPF), contact angle, air permeability, water permeability, antimicrobial activity for four types of microbes namely Pseudomonas aerugenosa (P), Staphylococcus aureus (S), Aspergillus niger (A) and Candida albicans (C), tensile strength and elongation at break, scanning electron microscopy (SEM) and FT-IR. Cellulosic fabrics with three constructions were used in this research (Shibeka Hunycomb and Crepe). The results showed that fabrics treated with chitosan gave the best result for resistance to microbes and UPF and this led to reduction in air permeability and water absorbance regardless of the type of construction of the treated fabric. From the above, these fabrics can be used in the various purposes such as medical field and protection fabrics from UV rays.