Bacillus coagulans is a probiotic bacterium with high beneficial effects on human health. The present research was performed to assess the antimicrobial effects of bacteriocin extracted from B. coagulans IS2 against different food-borne bacteria. B. coagulans IS2 was cultured on tryptic soy broth and incubated for 24 h at 37°C. Then, bacteria were sub-cultured on Man, Rogosa, Sharpe (MRS) broth. Produced bacteriocin was extracted from the MRS culture of B. coagulans IS2 by 70% ammonium sulphate. Escherichia coli, Salmonella enterica, and Listeria monocytogenes were cultured on Mueller Hinton Agar. Then, two separate 6 mm wells were created and filled with 200 µL of extracted bacteriocin. Presence of growth inhibition zones around wells weas considered as antimicrobial effect. The molecular weight of the bacteriocin was assessed by the Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The SDS-PAGE analysis showed presence of low molecular weight protein (