117418

Study the Thorax Ossification Centers in Partridge Embryos by Alizarin Red and Alcian Blue Double Staining Techniques

Article

Last updated: 01 Jan 2025

Subjects

-

Tags

Physiology & Animal Nutrition

Abstract

The current study was aimed to determine the onset of vertebral ossification, ribs, and sternum, and to evaluate ossification centers using the alizarin red and alcian blue staining techniques in the partridge embryos. One-hundred fertilized eggs of the partridge were placed in a hatching machine. From day 8 of incubation, three egg samples were randomly selected daily for staining until hatching. After staining, ossification centers in various parts of thorax were evaluated by a stereomicroscope. Data from examination of different thoracic parts of the partridge embryos indicated that cartilaginous thoracic vertebrae was were formed in the embryo on the 8th day, but the ribs and sternum were not visible. Cartilaginous ribs were formed on the 9th day of embryo. A cartilaginous sternum began to form on the 10thday of embryo. On the 13th day, cartilaginous uncinate processes were observed at the caudal border of embryonic ribs. From the 16th day, the first ossification center was detected in the body of ribs. The embryonic development process was progressive on the 17th and 18th days. The first ossification centers in the embryo of the 22nd and 23rd days were detectable in the cranio-lateral process of sternum and in the uncinate process of the first thoracic rib, respectively. On the 25th day, the cranio-medial process of sternum was the first ossification center in the embryos. The emergence of ossification centers in different parts of thorax and the developmental process were not observed at the pre-hatching time.

DOI

10.21608/ejvs.2020.38380.1184

Keywords

Ossification centers, Thorax, Partridge embryo, Alizarin red, Alcian blue

Authors

First Name

Masoud

Last Name

Sahimirad

MiddleName

-

Affiliation

Department of Basic Sciences and Hygiene, Science and Research Branch, Islamic Azad University, Tehran, Iran.

Email

dr.masoudsahimirad@gmail.com

City

-

Orcid

-

First Name

Hassan

Last Name

Gilanpour

MiddleName

-

Affiliation

Department of Basic Sciences and Hygiene, Science and Research Branch, Islamic Azad University, Tehran, Iran

Email

hgilanpour@yahoo.com

City

-

Orcid

-

First Name

Mohammadreza

Last Name

Paryani

MiddleName

-

Affiliation

Department of Basic Sciences, Karaj Branch, Islamic Azad university, Karaj, Iran

Email

parianymh@yahoo.com

City

-

Orcid

-

First Name

Bijan

Last Name

Radmehr

MiddleName

-

Affiliation

Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

Email

bradmehr42@yahoo.com

City

-

Orcid

-

First Name

Abbas

Last Name

Veshkini

MiddleName

-

Affiliation

Department of Clinical Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran

Email

drveshkiniabbas@gmail.com

City

-

Orcid

-

Volume

52

Article Issue

1

Related Issue

17187

Issue Date

2021-04-01

Receive Date

2020-08-07

Publish Date

2021-04-01

Page Start

39

Page End

46

Print ISSN

1110-0222

Online ISSN

2357-089X

Link

https://ejvs.journals.ekb.eg/article_117418.html

Detail API

https://ejvs.journals.ekb.eg/service?article_code=117418

Order

5

Type

Original Article

Type Code

140

Publication Type

Journal

Publication Title

Egyptian Journal of Veterinary Sciences

Publication Link

https://ejvs.journals.ekb.eg/

MainTitle

Study the Thorax Ossification Centers in Partridge Embryos by Alizarin Red and Alcian Blue Double Staining Techniques

Details

Type

Article

Created At

22 Jan 2023