SOLANUM nigrum L. was transplanted from soil to hydroponic culture system with Hoagland's solution to study its eco-physiological response to Cd and Ni toxicity. The plants were exposed to 10 or 25mg/L Cd or Ni over 7 days before harvesting for analysis. The findings of this study provided better understanding of stress adaptation in S. nigrum under Cd and Ni stress. The photosynthetic pigments and activities of antioxidant enzymes were estimated in the plant leaves. Chlorophyll content and carotenoids decreased progressively with increasing concentrations of both metals. Activities of the antioxidant enzymes POD, APX, CAT, and SOD in the leaves increased significantly by exposure to the high levels for both metals. The plant responses was varied with both metal and tested concentrations, reflecting the potentiality of using S. nigrum as phytoremediator of Cd and Ni polluted water in hydroponic culture system.