: Eight Accessions of grape (Vitis vinifera) are introduced and kept in vitro in the National Gene bank and Genetic Resources of Egypt. The objectives of this study were to determine optimal method for rapid clonal propagation of two local Egyptian cultivars (Khalili and Edkawy) using apices and nodal cutting stem dissected from 9 old month grape plants grown in green house taken from mature field growth plants. The effects of two different introduction media for the micropropagation of different grape cultivars were studied. A factorial treatment with  different combin-ations  using 3 concentrations of MS ( full,3/4 and half strength of MS ), 3 concentrations of  BAP (0.5 ,1.0,2.0 and 3.0 mg/ L) , 4 concentrations of  IAA( 0.1, 0.3, 0.5 and 1 mg / L) and one concentration of  TDZ (0.05 mg/L ), respectively were used for multiplication. The explants were in vitro rooted using medium supplemented with various combination and concentrations of MS, IAA, BAP and IBA, either with or without addition of charcoal. The best media for the multiplication rate of Khalili cultivar was achieved with ¾ MS supplemented with o.5 mg/L IAA and 1mg /L BAP, while the best medium for Edkawy cultivar was 1/2 MS supplemented with 0.5 mg /L BAP and 0.1 mg/L IAA. High root number  was achieved with media contained full strength of MS supplemented with 2 mg/L IAA and 0.5 mg /L BAP for Khalili cultivar, whereas the high root number for Edkawy cultivar was obtained by media included full strength of MS added with 3 mg/L IAA and 0.5 mg /L BAP. The rooted plantlets were acclimated to ex vitro conditions, most of the rooted shoots survived when transferred to pots containing Peat moss and Vermiculate (1:1).