Thirty-two bread wheat genotypes included two local cultivars, ten Egyptian selected line, and twenty advanced inbred line from (CIMMYT) were grown in two experiments, i.e. normal irrigation under water stress during the 2016/2017 and 2017/2018 seasons in Agronomy Dept. Research Station, Faculty of Agriculture, Assiut University. Mean squares due to irrigations and genotypes were significant for all traits i.e. plant height, spike length, number of spikes/row, number of spikelets/ spike, grain yield/row and 100 grain weight in each season and over the two seasons; suggesting that all traits were markedly affected by water stress and the presence of a wide range of differences among genotypes. The variance due to interaction between genotypes and irrigation (GxI) was significant for all studied traits. Mainly, the drought stress caused a relative loss in spike length, number of spikes/row, number of spikelets/spike, grain yield/row and 100 grain weight by 8.53, 23.62, 5.51, 35.81 and 10.83%, respectively, as average of two years.