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7568

Growth of Lactococcus lactis subsp lactis in Milk Under Control of Culture Acidity

Article

Last updated: 01 Jan 2025

Subjects

-

Tags

Dairy Science

Abstract

The effect of control of culture acidity on growth kinetics of one homofermentative strains of lactic acid bacteria, namely., Lactococcus lactis subsp lactis  was evaluated. The bacterial growth was estimated on the base of colony forming units, developed titratable acidity and incubation time .Sterilized skim milk was used as a medium with incubation at 34°C. Sampling had been carried out at zero time and each 2 hours intervals. After each sampling, a calculated volume of a liquid of 0.1 N sodium hydroxide was added to the culture to adjust the acidity of the remaining volume of the cultures to its initial value at zero time. The obtained results indicated that: 1-    When Lactococcus lactis subsp lactis grown under control of culture acidity lag phase was not observed. 2-    Significant effect of acidity control was noticed on the growth rate. Exponential phase of growth was between 2ed and 12th hour of incubation. However, the same culture being grown with control of culture acidity the exponential phase of growth was at interval of time from zero up to 24th hour of incubation. 3-      The maximum cell population in case of the control sample was 124 x 105 CFU /ml after 12 hours of incubation. The corresponding values when growth was under control of culture acidity was by a maximum cell population of 35x106 CFU/ml after 32 hour of incubation.

DOI

10.21608/ajas.2017.7568

Keywords

lactic acid bacteria, titratable acidity, colony count, culture acidity control, Mrs Media

Authors

First Name

Dalia

Last Name

Kamel

MiddleName

G.

Affiliation

Dairy Science Department, Faculty of Agriculture, Assiut University.

Email

-

City

Assiut

Orcid

-

First Name

Nanis

Last Name

Gomma

MiddleName

H.

Affiliation

Dairy Science Department, Faculty of Agriculture, Assiut University.

Email

-

City

Assiut

Orcid

-

First Name

Dina

Last Name

Osman

MiddleName

M.

Affiliation

Dairy Science Department, Faculty of Agriculture, Assiut University.

Email

-

City

Assiut

Orcid

-

First Name

A.

Last Name

Hassan

MiddleName

I.

Affiliation

Dairy Science Department, Faculty of Agriculture, Assiut University.

Email

-

City

Assiut

Orcid

-

Volume

48

Article Issue

6

Related Issue

961

Issue Date

2017-12-01

Receive Date

2017-11-20

Publish Date

2017-12-11

Page Start

32

Page End

39

Print ISSN

1110-0486

Online ISSN

2356-9840

Link

https://ajas.journals.ekb.eg/article_7568.html

Detail API

https://ajas.journals.ekb.eg/service?article_code=7568

Order

3

Type

Original Article

Type Code

62

Publication Type

Journal

Publication Title

Assiut Journal of Agricultural Sciences

Publication Link

https://ajas.journals.ekb.eg/

MainTitle

Growth of Lactococcus lactis subsp lactis in Milk Under Control of Culture Acidity

Details

Type

Article

Created At

22 Jan 2023