An experiment was conducted at the experimental farm of Agricultural Research station, Arab-El-Awamer, Assiut Governorate, Egypt, to evaluate the effect of bio-fertilizers in combination with different rates of organic manure on growth, yield and oil percentage of cumin (Cuminum cyminum) in the growing seasons of 2012/2013 and 2013/2014.The studied treatments included four rates of cattle manure as organic fertilizers; 0 (control), 12.5, 25, 37.5 m3/fed. were considered as main plots and four treatments of bio-fertilizers., 0 (without inculation), Azotobacter chroococcum(Az), Bacillus megatherium (Bm) and Bacillus circulans (Bc) as sub plots. The obtained results indicated that the studied characters were significantly increased with increasing the rate of organic fertilizer.The highest yield of fruits and oil percentage were obtained at 37.5 m3/fed. However, the combination between organic treatment and bio-fertilizers was significantly higher than organic manure alone, where the inoculation with bio-fertilizer Az or Bm combined with 37.5 m3/fed. of cattle manure improved the most growth parameters, yield and oil percentage compared to other treatments. The fertilization of plants with cattle manure 37.5 m3/fed.+Bc resulted in the highest of many components e.g., α-Thujene β-Pinene P-Cymene Phyllandrene and Cumin aldehyde recorded 1.664, 5.429, 11.489, 19.661and 32.623% respectevely So, applying 37.5 m3/fed.cattle manure combined with Azotobacter or Bacillus megatherium as bio-fertilizer significantly improved quantitative and qualitative yield of cumin and produced high quality product for human health