Shoot tip explants (0.5 cm) of four citrus rootstocks (trifoliate orange, sour orange, volkamariana and lime) were cultured on MS medium including vitamins supplemented with100 mg/l myo-inositol and 2.5 g/l gelrite. Three types of sugars (sucrose, fructose, glucose)were used at different concentrations (88, 132 and 176 mM) and two types of amino acids were used astryptophan at 0, 50, 75 and100 µM and glutathione at 0, 5, 10 and 20 µM. Growth regulators were used during multiplication and rooting stages as follow: a) multiplication,BAP at 1.0 mg/l and b) rooting, IBA at 0.5 mg/l. Sucrose was most effective on shoot and internode length and roots/shoot butthe number of shoots/explant, number of nodes and rooting percentage were more pronounced on the medium containing glucose, while fructose was more effective on the root length. Concerning amino acids, generally tryptophan was more effective on vegetative and rooting growth compared to glutathione.