Twenty three fusarial isolates of 3 species e.g. Fusarium oxysporum, F. solani and F. moniliforme which isolated  from diseased chickpea collected from different fields located at Assiut governorate were tested for their pathogenicity to chickpea. All these isolates caused pre and post emergence damping-off and root rot. F. oxysporum isolate (No.35) gave the highest percentage of infection. Isolates of Trichoderma harzianum, Pseudomonas fluorescence and Bacillus subtilis which isolated from chickpea rhizosphere inhibited growth of F. oxysporum, F. solani and F. moniliforme, in vitro and reduced pre and post emergence damping-off and root rot under greenhouse conditions. The selected antagonists varied in their inhibitory effect on radial growth of the tested pathogen and the reduction of the diseases.