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242243

The Role of Thaxtomin A and Necrosis Protein in Virulence of Streptomyces scabies that Cause Potato Common Scab Disease

Article

Last updated: 01 Jan 2025

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Subjects

-

Tags

Plant diseases

Abstract

Three isolates of Streptomyces scabies (St.1, St.2, and St.3) were examined for virulence on potato mini tubers, necrosis initiation on potato slices, inhibition of radish seedling length, production of thaxtomin A, and the presence of thaxtomin A (txtA) and nec1 genes. The three isolates, St.1, St.2, and St.3, were classified as highly virulent, moderately virulent, and weakly virulent, respectively. The St.1 isolate showed the highest scab severity index on potato mini tubers and necrosison potato slices, while the St.3 had the lowest, and St.2 had intermediate values. The St.1reduced radish seedling length by 82.35% and produced the highest toxin level (3.1 mg/ml), while the St.3 isolate reduced seedling length by 63.5% and produced the lowest level of toxin (1.18 mg/ml). The three isolates were positive for the presence of thaxtomin A (txtA) gene and produced a specific gene band at 500bp. However, St.1 and St.2 isolates were able to create a particular band at 700bp associated with thenecrosis gene (nec1), while the isolate St.3 lacked this band. Based on the total protein profile analysis, the three S. scabies isolates clustered into two groups which diverged at a similarity index of 0.78. The St.2 and St.3 virulent isolates were located in the same group with 0.86 similarity, while the highly virulent isolate St.1 was located in a separate group.Current findings highlight the correlation between the presence of thaxtomin A and necrosis virulence factors and S. scabies pathogenicity.
 

DOI

10.21608/asejaiqjsae.2022.242243

Keywords

potato common scab, Streptomyces scabies, thaxtomin A, nec1 gene

Authors

First Name

Asia

Last Name

Eid

MiddleName

R

Affiliation

Plant Pathology Department, Agriculure faculty, Damanhor University, Egypt

Email

asia.rashad@agr.dmu.edu.eg

City

-

Orcid

-

First Name

Shokry

Last Name

Bayoumi

MiddleName

Ramadan

Affiliation

Division of Genetics, Plant Pathology Dept., Faulty of Agriculture, Damanhour University

Email

shokry_bayoumi@agr.dmu.edu.eg

City

Damanhour

Orcid

0000-0003-1694-9512

First Name

Eman

Last Name

El-Argawy

MiddleName

-

Affiliation

Plant Pathology Department, Faculty of Agriculture, Damanhour University, Egypt

Email

eman.elargawy@agr.dmu.edu.eg

City

Damanhour

Orcid

-

First Name

Mahmoud

Last Name

Ghozlan

MiddleName

Helmy

Affiliation

Plant Pathology Department, Faculty of Agriculture, Damanhour University, Egypt

Email

mahmoud.ghozlan@agr.dmu.edu.eg

City

Damanhour

Orcid

-

Volume

43

Article Issue

2

Related Issue

32901

Issue Date

2022-04-01

Receive Date

2022-05-15

Publish Date

2022-04-01

Page Start

289

Page End

299

Print ISSN

1110-0176

Online ISSN

2536-9784

Article File

ASEJAIQJSAE_Volume 43_Issue 2_Pages 289-299.pdf

PDF . 601.1kB

Link

https://asejaiqjsae.journals.ekb.eg/article_242243.html

Detail API

https://asejaiqjsae.journals.ekb.eg/service?article_code=242243

Order

11

Type

Original Article

Type Code

53

Publication Type

Journal

Publication Title

Alexandria Science Exchange Journal

Publication Link

https://asejaiqjsae.journals.ekb.eg/

MainTitle

The Role of Thaxtomin A and Necrosis Protein in Virulence of Streptomyces scabies that Cause Potato Common Scab Disease

Details

Type

Article

Created At

22 Jan 2023