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213784

Relationship of Polygalacturonase Enzyme Activity between Brown Rot Isolates and their Virulence on Potato Cultivars

Article

Last updated: 01 Jan 2025

OverviewSimilar Items

Subjects

-

Tags

Plant diseases

Abstract

Ralstonia solanacearum, the bacterium that causes bacterial wilt in many plant species, produces a number of extracellular plant cell wall-degrading enzymes that are thought to be virulence factors. Endopolygalacturonase (endo-PG), PehA, and two exo-PGs, pehB and pehC, are among them. R. solanacearum was isolated and identified from sick potato tubers, yielding five isolates (BR1, BR2, BR3, BR4, and BR5). The pathogenicity of the isolates was determined, results revealed that the isolates differed in their virulence. R. solanacearum isolates (BR3, BR2, and BR5) had different levels of virulence on the potato stems of the examined cultivar (strong, moderate, and weak)respectively, so these isolates were chosen to assess potato cultivars for susceptibility to R. solanacearum isolates. R. solanacearum susceptibility differed amongst potato varieties. Spunta cultivar was the most sensitive, whereas Nicola cultivar was the most resistant. Mondial was a fairly sensitive cultivar. The activity of the PG enzyme was measured in the five isolates. BR3, BR2 and BR5 isolates were high, moderate and low activity, respectively. The optimum PG activity was observed at pH 5 and 5.5 and PG activity reached the highest level at 2 and 2.5 from incubation time, the maximum activity of PG was observed at 30 ºC and 40 ºC of incubation temperature, the optimum PG activity was observed with NaCl followed by CaCl2, The optimum NaCl concentrations for PG activity were 0.1 and 0.15 M
 

DOI

10.21608/asejaiqjsae.2021.213784

Keywords

pathogenicity, PG, Optimum, Sensitive, incubation, Cultivar

Authors

First Name

Asia

Last Name

Eid

MiddleName

R

Affiliation

Plant Pathology Department, Agriculure faculty, Damanhor University, Egypt

Email

asia.rashad@agr.dmu.edu.eg

City

-

Orcid

-

First Name

Sawsan

Last Name

El-shamy

MiddleName

S

Affiliation

Plant pathology department, Agriculture faculty, Damanhour university, Egypt

Email

sawsan.elshamy@agr.dmu.edu.eg

City

-

Orcid

-

Volume

42

Article Issue

4

Related Issue

28123

Issue Date

2021-10-01

Receive Date

2021-11-12

Publish Date

2021-12-30

Page Start

1,025

Page End

1,036

Print ISSN

1110-0176

Online ISSN

2536-9784

Article File

ASEJAIQJSAE_Volume 42_Issue 4_Pages 1025-1036.pdf

PDF . 571.2kB

Link

https://asejaiqjsae.journals.ekb.eg/article_213784.html

Detail API

https://asejaiqjsae.journals.ekb.eg/service?article_code=213784

Order

21

Type

Original Article

Type Code

53

Publication Type

Journal

Publication Title

Alexandria Science Exchange Journal

Publication Link

https://asejaiqjsae.journals.ekb.eg/

MainTitle

Relationship of Polygalacturonase Enzyme Activity between Brown Rot Isolates and their Virulence on Potato Cultivars

Details

Type

Article

Created At

22 Jan 2023