Beta
ManageSearchChatDiscover
46830

Detection of Milk Clotting Enzyme Produced by Lactic Acid Bacteria Isolated from Fermented Food

Article

Last updated: 01 Jan 2025

OverviewSimilar Items

Subjects

-

Tags

Dairy science

Abstract

Lactic acid bacteria (LAB) are known to produce extracellular enzymes that could be exploited for use in the dairy industry. Two LABs isolated from fermented foods   (paste shrimp) were evaluated for extracellular proteinase production, milk clotting activity (MCA) and proteolytic activity (PA). The two isolates were identified by (API 50CHL) as Pediococcus acidilactici SH, and Lactobacillus paracasei CF1. These LABs showed proteolytic activity using skim milk agar as indicated by the visible clear zones developed in skim milk agar. P. acidilactici SH showed highMCA 50 SU/ml with PA 1.9 U/ml while L. paracasei CF1 showed MCA 44 SU/ml) with PA 3.5 U/ml using trypticase peptone as a nitrogen source in enzyme production media. MCA was not observed when the LABs were grown in MRS broth. This study indicates the possibility of exploiting LAB from food sources for the production of milk-clotting enzymes for dairy production.
 

DOI

10.21608/asejaiqjsae.2019.46830

Keywords

Lactic acid bacteria (LAB) are known to produce extracellular enzymes that could be exploited for use in the dairy industry. Two LABs isolated from fermented foods (paste shrimp) were evaluated for extracellular proteinase production, milk clotting activity (MCA) and proteolytic activity (PA). The two isolates were identified by (API 50CHL) as Pediococcus acidilactici SH, and Lactobacillus paracasei CF1. These LABs showed proteolytic activity using skim milk agar as indicated by the visible clear zones developed in skim milk agar. P. acidilactici SH showed high MCA 50 SU/ml with PA 1.9 U/ml while L. paracasei CF1 show, Lactobacillus paracasei, Pediococcus acidilactici, Milk clotting enzyme, proteolytic activity

Authors

First Name

Mohamed

Last Name

Ahmad

MiddleName

Muftah

Affiliation

Faculty of Agriculture sebha University, Libya.

Email

-

City

-

Orcid

-

First Name

Zaiton

Last Name

Hassan

MiddleName

-

Affiliation

Faculty of Science and Technology, Universiti Sains Islam Malaysia (USIM)

Email

-

City

-

Orcid

-

Volume

40

Article Issue

July-September

Related Issue

6935

Issue Date

2019-08-01

Receive Date

2019-07-24

Publish Date

2019-08-01

Page Start

415

Page End

418

Print ISSN

1110-0176

Online ISSN

2536-9784

Article File

ASEJAIQJSAE_Volume 40_Issue July-September_Pages 415-418.pdf

PDF . 224.6kB

Link

https://asejaiqjsae.journals.ekb.eg/article_46830.html

Detail API

https://asejaiqjsae.journals.ekb.eg/service?article_code=46830

Order

3

Type

Original Article

Type Code

53

Publication Type

Journal

Publication Title

Alexandria Science Exchange Journal

Publication Link

https://asejaiqjsae.journals.ekb.eg/

MainTitle

Detection of Milk Clotting Enzyme Produced by Lactic Acid Bacteria Isolated from Fermented Food

Details

Type

Article

Created At

22 Jan 2023