Two field experiments  were conducted in the two  locations (Marawaa  and  in El-wasiata) situated at 21° 24´ and  21° 39´ N ;32° 20`and 32° 47´ E and 508, 185m over sea altitude for the 2 situates, respectively, during 2014/2015 season to study the response of  the six rows  barley variety  rehan  sown by the rate 70 kgha^-1 in lines 15cm apart to the nitrogen levels (0,50,100&150Kg N/ha^-1) in the form of urea 46%. The experiments were carried out  in RCBD with 4 replicates, plat area was (25m²). The  Results  revealed  significant (P<0.01) effect on  plant  height in El-wasiata ,number of economic tillers and spike traits in both locations ,where  the  greatest incase resulted from  150Kg N/ha^-1 compared to the control. Biological yield was significantly (P<0.01) affected by N-levels, the least yield (3.65, 3.76 t/ha) due to control however  the  highest (4.11, 4.63 t/ha) resulted from 150Kg N/ha^-1 application in the first and second   locations, respectively. Although there were increases  in grain yield from 1.46, 1.51 t/ha  due to non fertilization to 1.87, 1.96  t/ha  due to 150Kg  N/ ha application, but these differences  did not reach the significance level in both location, .Straw yield exhibited  significant (P<0.01) increases from ( 2.11, 2.24 t/ha)  in control treatment to (2.4, 2.5 t/ha)  at 150KgN/ha application . Harvest  index was not  significantly  affected by N-levels in both sites, while 1000 – grain weight  affected  significantly (P<0.01)where the  least values (35.86, 35.20g)  due to 50Kg N/ha^-1 and  zero  comparing to the highest (39.20, 37.40g) resulted from 150Kg N/ha in both Marawaa and El-wasiata, respectively.