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29981

Micropropagation and Somatic Embryogenesis Induction of Gardenia jasminoides Plants

Article

Last updated: 01 Jan 2025

OverviewSimilar Items

Subjects

-

Tags

Ornamental plants

Abstract

Gardenia jasminoides Ellis is an evergreen plant with fragrant creamy white flowers and glossy, darkgreen leaves, belongs to family Rubiaceae and member of the genus Gardenia. The current study aimed to afford a new technique for in vitro propagation and callus induction of G. jasminoides by using single nodes excised from soft cuttings using full strength MS salts supplemented with 30g/l sucrose, 4g/l gelrite and different concentrations of plant growth regulators. Generally, the current study showed that NAA and BA at 0.0 and 1.0 mg/l, respectively gave rise to the best results for initiation stage. Meanwhile, fortified the basal MS medium with BA and NAA at 2.0 and 0.250 mg/l, consecutively; resulted in the best results with respect to multiplication stage. Regarding rhizogenesis, the neoformed shoots produced from vegetative multiplication stage were rooted successfully upon cultured on MS medium supplemented with 1.0 mg/l IBA and NAA at either 0.250 or 0.500 mg/l, whereas the highest number of roots was recorded besides callus induction. Regarding embryonic callus induction, adding 2,4-D at 1.0 mg/l; enhanced embryonic callogenesis. Neoformed plantlets were acclimatized ex vitro and in vivo vigorously in mixture of perlite and peatmoss at (1:1, v/v), which resulted in the highest percentage value of survival / percent (100%) and successfully flowered and showed true-to-type plants ex vitro.
 

DOI

10.21608/asejaiqjsae.2019.29981

Keywords

Gardenia jasminoides, Rubiaceae, tissue culture, Plant growth regulators, embryonic callus

Authors

First Name

Mohamed K.

Last Name

Gaber

MiddleName

-

Affiliation

Plant Production Department, Faculty of Agriculture Saba Basha,Alexandria University, Alexandria, Egypt.

Email

m_kadry@alexu.edu.eg

City

-

Orcid

-

First Name

Ahmed A.

Last Name

Barakat

MiddleName

-

Affiliation

Botanical Garden Res. Dept. Hort. Res. Inst. Agric. Res. Center, Alexandria, Egypt.

Email

-

City

-

Orcid

-

Volume

40

Article Issue

JANUARY- MARCH

Related Issue

4685

Issue Date

2019-01-01

Receive Date

2019-03-05

Publish Date

2019-03-31

Page Start

190

Page End

202

Print ISSN

1110-0176

Online ISSN

2536-9784

Article File

ASEJAIQJSAE_Volume 40_Issue JANUARY- MARCH_Pages 190-202.pdf

PDF . 569.6kB

Link

https://asejaiqjsae.journals.ekb.eg/article_29981.html

Detail API

https://asejaiqjsae.journals.ekb.eg/service?article_code=29981

Order

16

Type

Original Article

Type Code

53

Publication Type

Journal

Publication Title

Alexandria Science Exchange Journal

Publication Link

https://asejaiqjsae.journals.ekb.eg/

MainTitle

Micropropagation and Somatic Embryogenesis Induction of Gardenia jasminoides Plants

Details

Type

Article

Created At

22 Jan 2023