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25836

Soybean Field Evaluation for Resistance to Cotton Leaf Worm (Spodoptera Littoralis) Confirmed by SSR Markers

Article

Last updated: 01 Jan 2025

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Tags

Crop genetics and breeding

Abstract

Insect resistant soybean {Glycine max (L.) Merr.} cultivars could reduce pesticide use in controlling insects, resulting in less risk to the environment. This study was conducted to develop an effective field and SSR analysis screening procedurs to screen 15 soybean genotypes . A field experiment was conducted for their resistance , and showed that  genotypesH105 andH153 recorded the highest insect resistance and low defoliation rating of 0.0 and 0.1 respectively, followed by H10L10A, L162, and H1L1. Genotype AGS-129 showed highest insect susceptibility and high defoliation rating of 4.0. Genotypes H10L10A, H 153, and H1L1 recorded the highest seed yield values due to the number of branches plant, number of pods plant, and 100-seed weight indicating that these genotypes could be recommended particularly with  high resistance to cotton leaf worm. A total of 13 SSR primer pairs were used to generate 39 bands (alleles). Nearly 92% (12) of the primer pairs amplified SSR alleles of expected size and 11 SSR loci were polymorphic. The polymorphic SSRs were successfully used to differentiate among 15 genotypes. These markers could be particularly useful for genetic differentiation cotton leaf worm resistance.

DOI

10.21608/asejaiqjsae.2019.25836

Keywords

defoliation, Cotton leaf worm, genotypic variance, soybean resistance for cultivars, SSR

Authors

First Name

Ahmed M.

Last Name

Serag

MiddleName

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Affiliation

Department of Genetics and Genetic Engineering , Faculty of Agriculture, Benha University, Egypt.

Email

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City

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Orcid

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First Name

Akrm R.

Last Name

Morsy

MiddleName

-

Affiliation

Legumes Department, Institute of Filed Crops Research, Agricultural Research Center, (ARC) Egypt.

Email

-

City

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Orcid

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First Name

Mona.A

Last Name

Farid

MiddleName

-

Affiliation

Department of Genetics, Faculty of Agriculture, Kafr Elsheikh University, 33516 – Kafr Elsheikh, Egypt.

Email

-

City

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Orcid

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Volume

40

Article Issue

JANUARY- MARCH

Related Issue

4685

Issue Date

2019-01-01

Receive Date

2018-11-27

Publish Date

2019-03-30

Page Start

1

Page End

11

Print ISSN

1110-0176

Online ISSN

2536-9784

Article File

ASEJAIQJSAE_Volume 40_Issue JANUARY- MARCH_Pages 1-11.pdf

PDF . 625.5kB

Link

https://asejaiqjsae.journals.ekb.eg/article_25836.html

Detail API

https://asejaiqjsae.journals.ekb.eg/service?article_code=25836

Order

1

Type

Original Article

Type Code

53

Publication Type

Journal

Publication Title

Alexandria Science Exchange Journal

Publication Link

https://asejaiqjsae.journals.ekb.eg/

MainTitle

Soybean Field Evaluation for Resistance to Cotton Leaf Worm (Spodoptera Littoralis) Confirmed by SSR Markers

Details

Type

Article

Created At

22 Jan 2023