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The Antimicrobial Activity of Chitosan and Its Application on Kariesh Cheese Shelf Life

Article

Last updated: 01 Jan 2025

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Tags

Dairy science

Abstract

Chitosan is a natural and non-toxic polysaccharide with many biological applications, largely known for its activity against a wide range of microorganisms, particularly as an antimicrobial agent.  in the present study, antimicrobial properties of two kind of commercial chitosan ( High (CSH) and low (CSL) molecular weight Chitosan) were evaluated, against Staphylococcus aureus, Escherichia coli, Lactobacillus delbruekii spp. bulgaricus , and Lactococcus lactis spp. Cremoris (LAB)as model bacteria besides isolated yeast culture from kariesh cheese surface  in vitro was carried out using standard  method  (broth dilution method). The antimicrobial activities of chitosan were explored by calculation of the Minimum Inhibitory Concentration (MIC) for the tested culture in media supplemented with various concentrations lying between 0.0004% (4 μg/ml) and 0.02% (200 μg/ml). The results revealed that E.coli and S. aureus were more sensitive in CSL (0.001, 0.0008% respectively) than in CSH 0.006%. While the MIC of LAB strains (Lb. bulgaricus and Lc. cremoris) were higher than the previous strains (0.01% and 0.02%). Adding CSL to the milk used in Kariesh cheese manufacturing by 0.03 and 0.05% level of CSL which previously inoculated with E. coli, S. aureus and yeast showed a significant decreased in the log CFU/g of E. coli, S. aureus and yeast in cheese which slightly decreased during storage period at 4oC for 10 days compared with other treatment without chitosan. On other hand addition of chitosan led to keep survival of LAB during cheese making and storage at 4oC. It could be concluded that the chitosan concentration dependence antibacterial activity with variation against several pathogenic bacterial strains which indicates their possibility to be used as antimicrobial agents in dairy products.
 

DOI

10.21608/asejaiqjsae.2017.4183

Keywords

Chitosan, The antimicrobial activity, kariesh cheese, MIC

Authors

First Name

Mahmoud.M.

Last Name

El-Dahma

MiddleName

-

Affiliation

Department of Dairy Res., Food Techn. Res. Institute, Agric. Res., Center, Giza, Egypt.

Email

-

City

-

Orcid

-

First Name

Ali.A.

Last Name

Khattab

MiddleName

-

Affiliation

Dairy Science &Technologies Dept., Faculty of Agriculture, El-Shatby, Alexandria University, Egypt.

Email

-

City

-

Orcid

-

First Name

Effat

Last Name

Gouda

MiddleName

-

Affiliation

Dairy Science &Technologies Dept., Faculty of Agriculture, El-Shatby, Alexandria University, Egypt.

Email

-

City

-

Orcid

-

First Name

Khaled.M.

Last Name

El-Saadany

MiddleName

-

Affiliation

Dairy Science &Technologies Dept., Faculty of Agriculture, El-Shatby, Alexandria University, Egypt

Email

-

City

-

Orcid

-

First Name

Wahed.A.

Last Name

Ragab

MiddleName

-

Affiliation

Department of Dairy Res., Food Techn. Res. Institute, Agric. Res., Center, Giza, Egypt.

Email

-

City

-

Orcid

-

Volume

38

Article Issue

October-December

Related Issue

694

Issue Date

2017-10-01

Receive Date

2017-10-04

Publish Date

2017-10-01

Page Start

733

Page End

745

Print ISSN

1110-0176

Online ISSN

2536-9784

Article File

ASEJAIQJSAE_Volume 38_Issue October-December_Pages 733-745.pdf

PDF . 539.0kB

Link

https://asejaiqjsae.journals.ekb.eg/article_4183.html

Detail API

https://asejaiqjsae.journals.ekb.eg/service?article_code=4183

Order

9

Type

Original Article

Type Code

53

Publication Type

Journal

Publication Title

Alexandria Science Exchange Journal

Publication Link

https://asejaiqjsae.journals.ekb.eg/

MainTitle

The Antimicrobial Activity of Chitosan and Its Application on Kariesh Cheese Shelf Life

Details

Type

Article

Created At

22 Jan 2023