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An Efficient Method for Regeneration of Stevia rebaudiana (Bertoni) in Egypt

Article

Last updated: 01 Jan 2025

OverviewSimilar Items

Subjects

-

Tags

Crop genetics and breeding

Abstract

Stevia rebaudiana Bertoni, a natural sweetener plant with zero calorie content, becomes an inevitable alternative to sugar especially with the over 346 million diabetic population across the world. The present experiments were conducted to standardize an in vitro culture technique of stevia rebaudiana to explore its potential for callus culture. Leaves explants were cultured on MS medium with specific concentration of growth regulators (2,4-D,BA, Kin and NAA). The results showed that, 1. MS medium containing 1mg/l 2,4-dichlorophexoy acetic acid (2,4-D)+0.5mg/l Benzyl adenine(BA) was gave the best result of callusing. 2. Higher regeneration of plants (32.6 plantlets/callus) was obtained by placing callus on MS medium with 5mg/l BA. 3. Highest rate of root formation (56%) and rooting average (6.1) was recorded in MS medium with 1mg/l IBA.

DOI

10.21608/asejaiqjsae.2012.3166

Keywords

Key words: stevia rebaudiana, in Vitro, Regeneration, Callus

Authors

First Name

Marwa

Last Name

M. Ghallab

MiddleName

-

Affiliation

Genetics and Breeding Department, Sugar Crop Research Institute, Sabahia Research Station

Email

-

City

-

Orcid

-

First Name

M. S.

Last Name

Saleh

MiddleName

-

Affiliation

Genetics and Breeding Department, Sugar Crop Research Institute, Sabahia Research Station

Email

-

City

-

Orcid

-

Volume

33

Article Issue

October-December

Related Issue

553

Issue Date

2012-10-01

Receive Date

2012-11-18

Publish Date

2012-12-30

Page Start

285

Page End

291

Print ISSN

1110-0176

Online ISSN

2536-9784

Article File

ASEJAIQJSAE_Volume 33_Issue October-December_Pages 285-291.pdf

PDF . 501.2kB

Link

https://asejaiqjsae.journals.ekb.eg/article_3166.html

Detail API

https://asejaiqjsae.journals.ekb.eg/service?article_code=3166

Order

10

Type

Original Article

Type Code

53

Publication Type

Journal

Publication Title

Alexandria Science Exchange Journal

Publication Link

https://asejaiqjsae.journals.ekb.eg/

MainTitle

An Efficient Method for Regeneration of Stevia rebaudiana (Bertoni) in Egypt

Details

Type

Article

Created At

22 Jan 2023