The objective of this study was to investigate the effect of broccoli intake on hyperglycemic rats.  The Broccoli was bought from the local market of Saudi Arabia and the chemical analyses were conducted. After steam boiling of broccoli for 4-5 minutes, it was dried at 50 centigrade and added to the diet. The research sample included 50 male adult Albino rats, its weight ranged from 200-210 grams. After the rats adapted to their new environmental conditions, they were divided into seven equal groups composed of 10 rats in each group. The first group was fed the standard diet (negative control group), whereas the 40 rats were injected with Streptozetocin 60 mg/kg and the rats became diabetic. The treated animals were divided into groups of the positive control group and the second group was fed with the standard meal; while the third, fourth, and fifth groups were fed with the standard meal mixed with 10, 20, 30% of dried broccoli respectively, for two mouths. At the end of the experiment, the results showed improved all biochemical parameters in the serum and antioxidants in each of the liver and kidneys of rats fed on broccoli.