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Effect of Detergent and Antibiotic Residuals on Proteolytic Activity of Plasmin on Beta Casein

Article

Last updated: 01 Jan 2025

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Tags

Dairy science

Abstract

Plasmin plays a significant role in cheese ripening and characteristics of dairy products. The antibiotic could be presented in milk as residuals from animal treated by antibiotic, while presence of detergent could be as residuals from cleaning of milking machines, tanks during transpirations, storage tanks and pasteurization equipments etc. The effect of detergents and antibiotic residuals on proteolytic action of plasmin on β-casein in model solution was determined by Urea-polyacrylamide gel electrophoresis. The results suggested that plasmin followed the proteolytic pathway on cow b-casein to produce g1, g2 and g3 as the degradation fragments.  The proteolytic pathway has been affected by presence of sodium hydroxide and nitric acid, while was not affected by presence of antibiotic. The results also  indicated that the presence of sodium hydrioxid increased the plasmin activity, while plasmin hydrolysate of B-casein slightly inhibited by nitric acid. The proteolytic activity of plasmin on b-casein was broader and higher at the end of incubation period, to yield low-sized fragments as evidenced with polyacrylamide gel electrophoresis.  Increased plasmin activity by sodium hydroxide residual could affect cheese making characteristics such as the rennet coagulation time, curd strength, and curd syneresis, produce off-flavor and bitterness in cheese. Since the plasmin is heat stable enzyme, increasing its activity in UHT milk could case gelatoin and produce off-flavor and bitterness.

DOI

10.21608/asejaiqjsae.2010.2310

Keywords

plasmin, b-casein, detergent and antibiotic residuals

Authors

First Name

Nahed

Last Name

Soliman

MiddleName

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Affiliation

Dairy Science and Technology Department, Faculty of Agriculture Alexandria University.

Email

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City

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Orcid

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First Name

Eman

Last Name

El Dakhakhny

MiddleName

-

Affiliation

Dairy Science and Technology Department, Faculty of Agriculture Alexandria University.

Email

-

City

-

Orcid

-

Volume

31

Article Issue

APRIL- JUNE

Related Issue

433

Issue Date

2010-04-01

Receive Date

2010-05-25

Publish Date

2010-06-30

Page Start

183

Page End

192

Print ISSN

1110-0176

Online ISSN

2536-9784

Article File

ASEJAIQJSAE_Volume 31_Issue APRIL- JUNE_Pages 183-192.pdf

PDF . 634.6kB

Link

https://asejaiqjsae.journals.ekb.eg/article_2310.html

Detail API

https://asejaiqjsae.journals.ekb.eg/service?article_code=2310

Order

6

Type

Original Article

Type Code

53

Publication Type

Journal

Publication Title

Alexandria Science Exchange Journal

Publication Link

https://asejaiqjsae.journals.ekb.eg/

MainTitle

Effect of Detergent and Antibiotic Residuals on Proteolytic Activity of Plasmin on Beta Casein

Details

Type

Article

Created At

22 Jan 2023