Textiles are common materials in health care requirements; therefore, hospitals laundering practices are critical to the prevention of transmission of pathogenic microorganisms, which could be the vector of many dangerous diseases. This study was conducted to study the microbial contamination of certain health care textile products in hospitals through both field and laboratory study. The field study revealed the presence of statistically significant relationship between microbial contamination of the hospital type, washing process, washer type, washing practice method, the amount of cleanser materials, as well as bleaching, disinfecting laundry, Ironing type, walls of the laundry, water source, sorting laundry, washing method used, the time of sterilization and storing the washed textiles. The laboratory study which was conducted on some textile products used in hospitals during Laundering processes indicated that difference in the microbial content before and after washing of bed linen was (59 %) (74 %); while the difference was (33 %) (24 %) in doctors' clothes and for the patients' garments, it was (56%) (30 %) .This may be due to the density of the threads and the weight per square meter (yar/m2). Microbial genera identified laundry on these textiles samples from different hospitals type revealed that gram negative was more persist laundry treatments and spread more than gram-positive bacteria. On the other hand, yeast species diminished completely after washing   and this indicated that the washing agents used had a positive impact on the presence of yeasts.