The high blood cholesterol is one of the reasons that increase the risk of heart disease, particularly atherosclerosis. This search aimed to study the effect of red grapes that rich with antioxidant for prevention of the dangers of high cholesterol in rats. The research sample included 24 adult healthy male rat. The samples were divided into four equal group. The first group was fed on a standard diet (control negative) and the second group was fed on a high cholesterol and fat diet (control positive); while the third and fourth groups took the control positive diet with the addition of grape juice as an alternative to drinking water by 50% and 100% respectively for 13 weeks. Blood lipid was determined and Histological study was done to the aorta, the data was statistically analyzed. The results showed that the grape juice decreased fat content in the blood and the aorta. The study recommended to intake grape juice as an antioxidant for the prevention of severity of vascular disease.