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Evaluation of Breeding Programs Susceptibility for Two Important Forage Crops Using DNA Barcoding

Article

Last updated: 01 Jan 2025

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Subjects

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Tags

Crop genetics and breeding

Abstract

This investigation was carried out to identify and evaluate Medicago sativa andTrifolium alexandrinum probability for breeding program based on two bar-coding genes (rbcl and Cox1 genes). Identification of Medicago sativa Baladi 1 was performed through rbcl and Cox1 genes. Medicago sativa Baladi 1 was identified as Medicago sativa voucher G00199095 ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit (rbcL) gene, partial cds; chloroplast (Sequence ID: KJ204375.1) and Medicago sativa voucher Ahrendsen_23 for rbcl and Cox1 genes respectively.
Identity values were recorded with 90% of identity for alfalfa, Baladi 1 Genotype ribulose – 1/5 – bisphosphate carboxylase / oxygenase large subunit (rbcl) gene (sequences ID: KJ206375.1) also, identity values were recorded with 91.24% of identity for alfalfa  Baladi 1 Genotype, cytochrome c oxiddase bubunit I gene (cox 1) (sequence ID: KJ 204375.1).
Trifolium alexandrinum Helaly genotype was identified as Trifolium alexandrinum (Sequence ID: HM850407.1) and Trifolium alexandrinum voucher K-016Hv (Sequence ID: KU234213.1) as rbcl and Cox1 genes respectively. Affiliation of genetic origin was detected for Trifolium alexandrinum with 100 % of similarity with origin source which indicate highly possibility for applying breeding programs comparing with Medicago sativa which reflect the lowest genetic similarity with origin source.

DOI

10.21608/asejaiqjsae.2017.1619

Keywords

DNA barcoding, rbcL, Cox 1, Trifolium alexandrinum, Medicago sativa, NCBI blast

Authors

First Name

Abdel

Last Name

Aziz.T. Bondok

MiddleName

-

Affiliation

Forage Crops Research Dept. Field Crops Res. Institute, Agriculture Research Center. Giza,Egypt.

Email

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City

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Orcid

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Volume

38

Article Issue

January-March

Related Issue

347

Issue Date

2017-03-01

Receive Date

2017-03-07

Publish Date

2017-03-01

Page Start

27

Page End

37

Print ISSN

1110-0176

Online ISSN

2536-9784

Article File

ASEJAIQJSAE_Volume 38_Issue January-March_Pages 27-37.pdf

PDF . 523.4kB

Link

https://asejaiqjsae.journals.ekb.eg/article_1619.html

Detail API

https://asejaiqjsae.journals.ekb.eg/service?article_code=1619

Order

4

Type

Original Article

Type Code

53

Publication Type

Journal

Publication Title

Alexandria Science Exchange Journal

Publication Link

https://asejaiqjsae.journals.ekb.eg/

MainTitle

Evaluation of Breeding Programs Susceptibility for Two Important Forage Crops Using DNA Barcoding

Details

Type

Article

Created At

22 Jan 2023