Background and Objectives: The centrosome-associated kinase aurora A (AURKA) is involved in genetic instability and is over-expressed in several human carcinomas including colorectal cancer (CRC). It plays a critical role in cancer cell development, proliferation, motility and survival. It is suggested that the Aurora kinase A expression in colorectal cancer significantly increase, which may play an important role in development of colorectal cancer.Design and Methods: In this study, the expression of AURKA gene was analyzed in 15 malignant patients, 13 premalignant patients and 23 control patients. AURKA gene expression was analyzed using a Real-Time Quantitative Reverse-Transcriptase Polymerase Chain Reaction (RTQ-PCR) to examine the copy number gains and mRNA level of AURKA in CRC.Results: AURKA gene expression was highly expressed in (33.3%, 5 of 15 CRC studied patients) with (mean 213.4, P value=0.005) indicated highly significant difference between control, premalignant and malignant groups. Conclusions: These findings indicated that colorectal cancers exhibit different mechanisms of aurora A regulation and this may impact the efficacy of aurora-A targeted therapies.